mouse embryonic fibroblasts  (ATCC)

Journal: bioRxiv

Article Title: AI-discovered protein fragments as generalizable regulators of biomolecular condensates

doi: 10.64898/2026.05.08.723928

Figure Lengend Snippet: ( A ) Mouse embryonic fibroblasts (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.

Article Snippet: Mouse embryonic fibroblast (MEF ATCC CRL-2645) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; high glucose, Invitrogen) supplemented with 10% fetal bovine serum (FBS, Thermo Scientific), 1% penicillin–streptomycin, and 2 mM Glutamax at 37°C in a humidified incubator with 5% CO2.

Techniques: Transfection, Expressing, Fluorescence, Imaging, Microscopy, Standard Deviation

Journal: Gels

Article Title: Tendon dECM Composited with Chitosan with Loading Skin Precursor Stem Cell Exosome for Enhanced Diabetic Wound Healing

doi: 10.3390/gels12050361

Figure Lengend Snippet: The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

Article Snippet: The cytocompatibility of dECM@QCS and exo@dECM@QCS composite hydrogels was investigated by culturing with mouse embryonic fibroblast cells (NIH 3T3, ATCC, CRL-1658TM, Manassas, VA, USA) using fluorescent images and Cell Counting Kit-8 (CCK-8) assay [ ].

Techniques: Activity Assay, Staining, Wound Healing Assay, Migration, CCK-8 Assay